Journal: Fundamental Research
Article Title: Apigenin alleviates osteoporosis by orchestrating SIRT1/HIF1α signaling in mesenchymal stem cells
doi: 10.1016/j.fmre.2024.02.002
Figure Lengend Snippet: API upregulated SIRT1 to stabilize HIF1α expression in the OBs. OP-BMSCs were differentiated into OBs. (a) ALP staining of OB after 7 d (b) Quantification of AKP activity ( n = 3). (c) ARS staining of OB after 14 d. (d) Quantification of ARS ( n = 3). (e) The protein expression of SIRT1, HIF1α and β-actin in the OB at 5 days. (f, g) Quantification of SIRT1 and HIF1α expression in OB after 5 d ( n = 3). (h) Images of femur SIRT1 IHC staining. (i) Quantification of SIRT1-positive cells staining ( n = 5).
Article Snippet: Fig 5 To explore the underlying mechanism of API improvement in bone metabolism, we introduced a specific inhibitor of SIRT1 (EX527, 1 μM; Selleck, China).
Techniques: Expressing, Staining, Activity Assay, Immunohistochemistry